hbmec cell line  (Innoprot Inc)

Journal: Frontiers in Microbiology

Article Title: Engineering the Single Domain Antibodies Targeting Receptor Binding Motifs Within the Domain III of West Nile Virus Envelope Glycoprotein

doi: 10.3389/fmicb.2022.801466

Figure Lengend Snippet: sdAb targeting the DIII blocked the interaction between rDIII and human brain microvascular endothelial cells (hBMECs) proteins. (A) NC strips transblotted with hBMEC proteins were incubated with either rDIII (positive control) or rDIII pretreated with sdAbs targeting receptor binding motifs of DIII. sdAb used to pretreat rDIII are mentioned on each NC strip. The interaction between rDIII/pre-treated rDIII and hBMEC proteins at ∼15 kDa was detected by HisProbe-HRP conjugate and chemiluminescent HRP substrate in Western blot analysis. sdAbs blocking the rDIII-hBMEC interaction showed the absence of chemiluminescent HRP signal at 15 kDa is framed. For negative control (Neg.), NC strip of hBMEC protein was precluded with the incubation of rDIII in the same Western blot assay, and no signal was detected. (B) The ability of sdAb A1 , sdAb A6 , sdAb A9 , and sdAb A10 to block the interaction between rDIII and hBMECs was detected through cell ELISA. rDIII alone (positive control) or aforestated sdAbs-treated rDIII was incubated on paraformaldehyde-fixed hBMECs on the ELISA plate. The interaction was detected by HisProbe-HRP conjugate and UltraTMB-ELISA substrate. hBMECs precluded with the addition of either rDIII or sdAbs served as a negative control. Antigens and sdAbs added on fixed hBMECs in ELISA plate (framed) are mentioned below each bar graph. A significant difference between the compared treatments by one-way ANOVA ( p < 0.05) is indicated by different alphabets.

Article Snippet: Human brain microvascular endothelial cells (hBMEC/D3 cell line; Merck/Millipore) were cultured in a T-75 cell culture flask (Sarstedt, Bratislava, Slovakia) following the protocol described by with minor modifications.

Techniques: Incubation, Positive Control, Binding Assay, Stripping Membranes, Western Blot, Blocking Assay, Negative Control, Enzyme-linked Immunosorbent Assay

Journal: Viruses

Article Title: Densovirus Oil Suspension Significantly Improves the Efficacy and Duration of Larvicidal Activity against Aedes albopictus

doi: 10.3390/v14030475

Figure Lengend Snippet: The infection and pathogenicity of AalDV-5 on non-target species.

Article Snippet: The cell lines, including human endothelial cell line (HBMEC), were purchased from ScienCell (Carlsbad, CA, USA), and human glioma cells (U251), baby hamster kidney cells (BHK-21), Drosophila melanogester (S2), and Spodoptera frugiperda (Sf9) cell lines were bought from Sigma-Aldrich (St. Louis, MO, USA).

Techniques: Infection, Concentration Assay, Injection